One hundred and twenty (15%) tissues from different organs had suspected bTB lesions at PM. A Prospective study was conducted on 800 cattle slaughtered in the three Zonal abattoirs of Bauchi State, Nigeria. This study was aimed at determining the prevalence of bovine tuberculosis in slaughtered cattle, based on PM meat inspection, Ziehl-Neelsen staining, and PCR techniques in Bauchi State, Nigeria. It also poses a public health threat and economic losses. Ibrahim, S.Д«ovine tuberculosis is a chronic, infectious, and contagious zoonotic disease of domestic animals, wild animals, and humans. Reducing the concentration of basic fuchsin below 0.3% in ZN staining was found to significantly reduce its sensitivity.Дetection of Mycobacterium bovis in Organs of Slaughtered Cattle by DNA-Based Polymerase Chain Reaction and Ziehl-Neelsen Techniques in Bauchi State, Nigeria The sensitivity of ZN using 0.3% (65%) and 1% basic fuchsin (62%) was comparable, while it was reduced using 0.1% (74%) compared to 1% basic fuchsin (83%). All samples underwent mycobacterial culture. The coded smears were read and discrepancies resolved. Another set of duplicate smears from 398 samples were stained with ZN, one with 1% basic fuchsin and the other 0.1%. To evaluate Ziehl-Neelsen (ZN) staining using variants of carbol-fuchsin solution, duplicate smears from 416 samples were stained with ZN, one set with 1% basic fuchsin and the other 0.3%. Selvakumar, N Sekar, M Gomathi Rahman, F Syamsunder, A Duraipandian, M Wares, F Narayanan, P R All rights reserved.Д¬omparison of variants of carbol-fuchsin solution in Ziehl-Neelsen for detection of acid-fast bacilli.

Copyright Г‚В© 2013 Hainan Medical College. CA-RT immunoassay is more valid and reliable than MZN-AF staining method. Out of a total of 85 suspected specimens, CA-RT detected 7(8.2%) but MZN-AF detected 6(7.1%) cases as positive. Based on the results, the SN, SP, AI, PPV and NPV were high in CA-RT than MZN-AF staining method, ie., 86.7%vs. However, CA-RT detected no positive case in 40 normal controls but MZN-AF detected 2(5%) as positive cases. Out of 15 positive controls, CA-RT detected 13 (86.7%) while MZN-AF detected 11(73.3%) positive cases. Validation parameters including sensitivity (SN), specificity (SP), accuracy index (AI), positive predictive value (PPV), and negative predictive value (NPV) were evaluated for both tests. All samples were subjected to CA-RT and conventional MZN-AF staining method. The study was conducted in the department of parasitology, central laboratory, Alnoor Specialist Hospital, Makkah, Saudi Arabia. A total of 85 stool samples were collected from suspected patients with cryptosporidiosis over 6 months during the period from January till June, 2011. Fifteen preserved stool samples from previously confirmed infections were used as positive controls and 40 stool samples from healthy people were used as negative control. To evaluate the validity of Crypto-Giardia antigen rapid test (CA-RT) in comparison with the conventional modified Ziehl-Neelsen acid fast (MZN-AF) staining method for the diagnosis of cryptosporidiosis. Zaglool, Dina Abdulla Muhammad Mohamed, Amr Khodari, Yousif Abdul Wahid Farooq, Mian Usman This work will improve the accuracy and practicability of the computer-aided diagnosis of tuberculosis.Д¬rypto-Giardia antigen rapid test versus conventional modified Ziehl-Neelsen acid fast staining method for diagnosis of cryptosporidiosis. Experimental results show that the single objects composing the touching objects are successfully segmented by the proposed method. The gradient vector flow snake is used to estimate the object edge. The skeletons of the objects are used for structure analysis based on the hierarchy tree. In this paper, we present a segmentation method of combining the hierarchy tree analysis with gradient vector flow snake to address this problem. Touching Mycobacterium tuberculosis objects in the Ziehl-Neelsen stained sputum smear images present different shapes and invisible boundaries in the adhesion areas, which increases the difficulty in objects recognition and counting. Segmentation of touching mycobacterium tuberculosis from Ziehl-Neelsen stained sputum smear images

Como cambiar multisim 14.1 a espaГ±ol